Alsever's Solution

Alsever's Solution

Reagents

· Citric acid C(OH)(COOH)(CH2.COOH)2.H2O	0.055g
· Sodium Citrate Na3C6H5O7.2H2O	0.8g
· D-Glucose C6H12O6	2.05g
· Sodium chloride NaCl	0.42g
· Distilled water to make up to 100 mL

Method

1.    Weigh out reagents into a conical flask.

2.    Dissolve of distilled water and make up to100 mL.

3.    Dispense into sterile 10 mL bottles.

4.    Sterilize by autoclaving at 116°C for 10 minutes. Use slow exhaust.

5.    Allow to cool, then tighten the lids and label the bottles.

6.    Store in the refrigerator.