Alsever's Solution

Alsever's Solution

Reagents

· Citric acid C(OH)(COOH)(CH2.COOH)2.H2O	0.055g
· Sodium Citrate Na3C6H5O7.2H2O	0.8g
· D-Glucose C6H12O6	2.05g
· Sodium chloride NaCl	0.42g
· Distilled water to make up to 100 mL

Method

1.    Weigh out reagents into a conical flask.

2.    Dissolve of distilled water and make up to100 mL.

3.    Dispense into sterile 10 mL bottles.

4.    Sterilize by autoclaving at 116°C for 10 minutes. Use slow exhaust.

5.    Allow to cool, then tighten the lids and label the bottles.

6.    Store in the refrigerator.

Phosphate buffered saline (PBS)

Reagents

·	Sodium chloride NaCl	40.0g
·	Potassium chloride KCl	1.0g
·	Potassium dihydrogen phosphate anhydrous KH2PO4	1.0g
·	Disodium hydrogen phosphate anhydrous Na2HPO4	4.6g
OR	Disodium hydrogen phosphate dihydrous Na2HPO4.2H2O	5.75g
OR	Disodium hydrogen phosphate dodecahydrous Na2HPO4.12H2O	11.6g
·	Distilled water to make up to 5 L

Method

1.    Weigh out the reagents and place in a 5 L conical flask.

2.    Add distilled water to make 5 L. Mix well.

3.    Check pH. Adjust to pH 7.3 to 7.4.

4.    Pour into storage bottles.

5.    Autoclave at 121°C for 15 minutes. Use a slow exhaust.

6.    Allow to cool, then tighten the lids and label the bottles.